Cloning vector system for Corynebacterium glutamicum
نویسندگان
چکیده
منابع مشابه
Cloning vector system for Corynebacterium glutamicum.
A protoplast transformation system has been developed for Corynebacterium glutamicum by using a C. glutamicum-Bacillus subtilis chimeric vector. The chimera was constructed by joining a 3.0-kilobase cryptic C. glutamicum plasmid and the B. subtilis plasmid pBD10. The neomycin resistance gene on the chimera, pHY416, was expressed in C. glutamicum, although the chloramphenicol resistance gene was...
متن کاملProduction system for biodegradable polyester polyhydroxybutyrate by Corynebacterium glutamicum.
A biosynthetic pathway for poly(3-hydroxybutyrate) [P(3HB)] production by Corynebacterium glutamicum was developed by introducing the phbCAB operon derived from Ralstonia eutropha. P(3HB) synthase activity was detected in this recombinant C. glutamicum carrying a cell surface protein gene promoter. Intracellular P(3HB) was microscopically observed as inclusion granules and its content was calcu...
متن کاملEngineering Corynebacterium glutamicum for violacein hyper production
BACKGROUND Corynebacterium glutamicum was used as a metabolic engineering chassis for production of crude violacein (mixture of violacein and deoxyviolacein) due to Corynebacterium's GRAS status and advantages in tryptophan fermentation. The violacein is a commercially potential compound with various applications derived from L-tryptophan. RESULTS Corynebacterium glutamicum ATCC 21850 that co...
متن کاملEmerging Corynebacterium glutamicum systems biology.
Corynebacterium glutamicum is widely used for the biotechnological production of amino acids. Amino acid producing strains have been improved classically by mutagenesis and screening as well as in a rational manner using recombinant DNA technology. Metabolic flux analysis may be viewed as the first systems approach to C. glutamicum physiology since it combines isotope labeling data with metabol...
متن کاملTryptophan-Producing Corynebacterium glutamicum Strain
The aromatic amino acids are synthesized via a common biosynthetic pathway. A tryptophan-producing mutant of Corynebacterium glutamicum was genetically engineered to produce tyrosine or phenylalanine in abundance. To achieve this, three biosynthetic genes encoding the first enzyme in the common pathway, 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase (DS), and the branch-point enzymes chor...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1985
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.162.2.591-597.1985